Journal:
Article Title: 5? Nuclease PCR Assay To Detect Yersinia pestis
doi:
Figure Lengend Snippet: Specificity of the Y. pestis TaqMan 5′ nuclease PCR assay
Article Snippet: Some were obtained from the American Type Culture Collection, Rockville, Md., and some were from strains and stocks maintained at the U.S. Army Medical Research Institute of Infectious Diseases, while others were obtained from clinical isolates obtained at the Frederick Memorial Hospital in Frederick, Md., and identified by the Microscan protocol. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species Isolate Ct a Aeromonas hydrophila M10915 #2 31.7 Bacillus anthracis Ames 30.6 B. anthracis Colorado 30.6 B. anthracis Vollum 40.0 Coxiella burnetii Ohio 40.0 Enterobacter aerogenes M8001 33.6 E. cloacae ATCC 49141 32.0 E. faecalis ATCC 49149 32.8 E. sakazakii M12662 40.0 Escherichia coli M127495 40.0 Haemophilus influenzae ATCC 10211 40.0 Klebsiella oxytoca ATCC 49131 31.7 K. pneumoniae ATCC 49472 35.4 Normal throat flora 40.0 Normal fecal flora 40.0 No-template control 40.0 Proteus aeruginosa ATCC 27853 40.0 P. mirabilis M12702 -1 40.0 Salmonella sp. (group B) M14666 40.0 Shigella sonnei M11098 37.4 Staphylococcus aureus ATCC 29247 33.7 S. agalactiae M10828 31.6 S. epidermidis ATCC 49134 33.6 Serratia marcescens M10759 40.0 Streptococcus pyogenes M10780 40.0 Vibrio cholerae N16961 -K004 31.7 Yersinia enterocolitica Hobs 40.0 Y. enterocolitica E268 40.0 Y. enterocolitica ATCC 9610 40.0 Y. pestis 195/P 15.5 Y. pestis A1122 22.0 Y. pestis Angola 18.3 Y. pestis CO92 18.4 Y. pestis Java-9 18.2 Y. pestis K25 14.7 Y. pestis Nairobi 15.0 Y. pestis Pestoides A 18.6 Y. pestis Pestoides Ba 17.6 Y. pestis Pestoides C 18.4 Y. pestis Pestoides D 22.2 Y. pestis Pestoides E 37.6 Y. pseudotuberculosis ATCC 6902 34.4 Y. pseudotuberculosis ATCC 6903 32.7 Y. pseudotuberculosis ATCC 6904 34.5 Open in a separate window a Ct, threshold cycle value as calculated by SDS 1.0.5d30 software.
Techniques: Control